Perfluorodecanoic acid-induced oxidative stress and DNA damage investigated at the cellular and molecular levels
By Mengchen Xu, Tong Zhang, Chao Lva, Qigui Niu, Wansong Zong, Jingchun Tang, Rutao Liu
Ecotox. and Envir. Safety
September 30, 2019
Perfluorodecanoic acid (PFDA) has been widely used in production of many daily necessities because of its special nature. Althoughtoxic effects of PFDA to organisms have been reported, there is little research on the genotoxicity induced by oxidative stress of PFDA on the cellular and molecular levels simultaneously. Thus, we investigated the DNA oxidative damage caused by PFDA in mouse hepatocytes. On the cellular level, an increase in ROS content indicated that PFDA caused oxidative stress in mouse hepatocytes. In addition, after PFDA exposure, the comet assay confirmed DNA strand breaks and an increased 8-OHdG content demonstrated DNA oxidative damage. On the molecular level, the microenvironment of aromatic amino acids, skeleton and secondary structure of catalase (CAT) were varied after PFDA exposure and the enzyme activity was reduced because PFDA bound near the heme groups of CAT. Moreover, PFDA was shown to interact with DNA molecule by groove binding. This study suggests that PFDA can cause genotoxicity by inducing oxidative stress both on the cellular and molecular levels.
• The work establishes a combined molecular and cellular toxicity evaluation method.
• PFDA causes DNA damage by inducing oxidative stress.
• Binding interactions of PFDA with CAT are systematically investigated.
• PFDA binds to the DNA molecule in a minor groove.
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