Suspended sediment exacerbates perfluorooctane sulfonate mediated toxicity through reactive oxygen species generation in freshwater clam Corbicula fluminea

By Liu, Yan, Muhammad Junaid, Peng Xu, Wei Zhong, Baozhu Pan, and Nan Xu
Env. Poll.
September 28, 2020
DOI: 10.1016/j.envpol.2020.115671

Perfluorooctane sulfonate (PFOS) potentially adsorbs on the surface of suspended sediment (SPS), which can develop a toxic “pool” bioavailable to benthic organisms. In this study, the freshwater clam Corbicula fluminea was employed as a zoobenthos model to study the effects of SPS (collected from the Yellow River) on the bioaccumulation and toxicity (from the molecular level to cellular and physiological levels) caused by PFOS exposure. Besides, the enhanced integrated biomarker response (EIBR) system was applied as an index to evaluate the in-depth toxic effects of PFOS and SPS single and co-exposure at various treatment levels. Our results demonstrated that PFOS-SPS co-exposure (at sub-lethal doses of PFOS) significantly increased the bioaccumulation of PFOS, and induced the elevated levels of reactive oxygen species (ROS), the significantly increased activities of superoxide dismutase (SOD) and catalase (CAT) enzymes, the significantly increased content of malondialdehyde (MDA), and the significantly upregulated expression levels of sod, selenium-dependent glutathione peroxidase (se-gpx), heat shock protein 22 (hsp22), heat shock protein 40 (hsp40) and cytochrome P450 30 (cyp30) genes. Further, the co-exposure induced the significantly higher histopathological alterations in the gonads and digestive glands, and even elevated the inhibition of siphoning behavior in clams. In addition, the EIBR index also revealed the highest values for PFOS and SPS co-exposure, compared to the individual SPS or PFOS exposure. The results indicated that at high levels of PFOS exposure (especially at 1000 μg/L), the presence of SPS might increase the generation of ROS by influencing the bioaccumulation of PFOS, which enhanced the toxicity of PFOS to C. fluminea. These results potentially provide basic information for the comprehensive evaluation of the toxic effects of PFOS on benthos in a multi-sediment river ecosystem.

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